Reporter
GG105

Part:BBa_K2145100:Design

Designed by: Samuel Clamons, Katie Maruna, Laura Chen, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)


GFP/RFP reporter (Forward/Forward) separated by three dCas clamp sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2020
    Illegal AgeI site found at 2132
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constructed with Golden Gate assembly.


Source

Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.

References